Isolation and Amino Acid Sequence Studies*

ثبت نشده
چکیده

Glucagon was isolated from a side fraction generated during the preparation of insulin and the new pancreatic peptide, avian pancreatic polypeptide from chicken pancreas. The immunological and biological properties are similar to those of beef-pork glucagon. The amino acid composition of chicken glucagon indicates that it contains 1 more serine residue than the porcine hormone and 1 less aspartic acid (asparagine) residue. Thus, chicken glucagon appears to be identical with turkey glucagon.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Isolation and Characterization of a New Peroxisome Deficient CHO Mutant Cell Belonging to Complementation Group 12

We searched for novel Chinese hamster ovary (CHO) cell mutants defective in peroxisome biogenesis by an improved method using peroxisome targeting sequence (PTS) of Pex3p (amino acid residues 1–40)-fused enhanced green fluorescent protein (EGFP). From mutagenized TKaEG3(1–40) cells, the wild-type CHO-K1 stably expressing rat Pex2p and of rat Pex3p(1–40)-EGFP, numerous cell colonies resistant to...

متن کامل

Isolation and molecular characterization of partial FSH and LH receptor genes in Arabian camels (Camelus dromedarius)

Very little is known about LHR and FSHR genes of domestic dromedary camels. The main objective of this study was to determine and analyze partial genomic regions of FSHR and LHR genes in dromedary camels for the first time. To this end, a total of 50 DNA samples belonging to dromedary camels raised in Iran were sent for sequencing (25 samples of each gene). We compared the nucleotide sequences ...

متن کامل

Isolation, Cloning and Sequence Analysis of 1-Aminocyclopropane-1-Carboxylate Deaminase Gene from Native Sinorhizobium meliloti

Background: Many plant growth-promoting bacteria including Rhizobia contain the 1-aminocyclopropane-1-carboxylate (ACC) deaminase enzyme that can leave ACC, and thereby lower the level of ethylene in stressed plants. Drought and salinity are the most common environmental stress factors for plants in Iran. Objectives: The main aim of this research was development of bio-fertilizers containing A...

متن کامل

Phylogenetic and sequence analysis of the growth hormone gene of two sturgeons, Huso huso and Acipenser Gueldenstaedtii

In this study, the cDNA Growth Hormone (cGH) of the Belugasturgeon (Husohuso) and Russian sturgeon (Acipensergueldenstaedtii) were cloned and sequenced, and phylogenetic relationships were examined using nucleic acid and amino acid sequences. The nucleotide sequence of the Beluga GH has an open reading frame of 645 nucleotides encoding a protein 214 amino acid residues. The signal peptide cleav...

متن کامل

Preliminary Report of NAD+-Dependent Amino Acid Dehydrogenase Producing Bacteria Isolated from Soil

Amino acid dehydrogenases (L-amino acid: oxidoreductase deaminating EC 1.4.1.X) are members of the wider superfamily of oxidoreductases that catalyze the reversible oxidative deamination of an amino acid to its keto acid and ammonia with the concomitant reduction of either NAD+, NADP+ or FAD. These enzymes have been received much attention as biocatalysts for use in biosensors or diagnostic kit...

متن کامل

Identification of a cDNA sequence coding for kruppellike factor 2b (Klf2b) from the skin mucosa of common carp (Cyprinus carpio)

Kruppel-like factors (Klfs) are a highly related zinc-finger family of transcription factors implicated inthe regulation of the eukaryotic cellular growth and differentiation of a diverse set of cells in mammal. UsingRT-PCR technique, a 456 bp cDNA fragment encoding N-terminus part of a Klf2b was isolated from the skinmucosa of common carp (Cyprinus carpio) using two degenerative oligonucleotid...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2002